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KMID : 0385520220350010024
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Analytical Science & Technology
2022 Volume.35 No. 1 p.24 ~ p.31
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Spectrofluorometric quantification of Clozapine in pharmaceutical formulations and human plasma
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Mohammed Ali Fahim
Alshirifi A. N.
Kadhim Kasim Hassan
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Abstract
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Herein, we present a simple, precise, accurate, and ultra-sensitive spectrofluorimetric method for estimation of clozapine (CLZ) in tablets and human plasma was developed and then validated. A highly fluorescent brown-yellowish fluorophore was formed (λex=469 nm, λemi=540 nm) as a nucleophilic substitution reaction occurred between CLZ and 4-chloro-7-nitro-2,1,3-benzoxadiazole (NBD-Cl) in alkaline mcllavine buffer (pH 9.0). Optimum values of experimental parameters were carefully determined and optimized. The calibration curve was rectilinear over the concentration range of 80-900 ng mL-1 with a linear correlation coefficient (r=0.9984). The LOD and LOQ were determined to be 14 ng mL-1 and 42 ng mL-1, respectively. The proposed approach has been used successfully to quantification of Clozapine in its commercial formulations and human plasma.
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KEYWORD
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clozapine, NBD-Cl, spectrofluorimetric, human plasma
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